Key deer can fall prey to:
- Puma (Puma concolor) – introduced or escapee
- Bobcat (Lynx rufus)
- Coyotes (Canis latrans)
- Domestic dogs (Canis lupus familiaris)
They are also threatened by invasive python populations, including the Burmese python (Python bivittatus) and reticulated python (Python reticulatus). These pythons can eat any size key deer they can swallow whole, regardless of the snake's or deer's age.